Figure 3. Transduction efficiency and RNA packaging of the PMM.psi transgene vectors.

A) Lentiviruses harvested from transfected 293FT cells with pLenti/PMM1.psi or PMM2.psi were used for MT4 transduction, and the transduced cells were observed at 72 hr post-transduction. B) Amounts of virus particles in p24 in harvested viral supernatants were measured by ELISA, and C) Percentage of EGFP positive MT4 cells transduced was determined by FACS. D) Lentiviral transduction ratio was calculated from FACS and p24 ELISA results. Four independent experiments were performed. E) qRT-PCR was performed in triplicate as described in the legend of Figure 2, and the data were presented as p24 normalized ratios. All data were compared to Lenti/EGFP control in One-way ANOVA (F-test), and data showing significant differences (p<0.001) were marked with three asterisks.