Skip to main content
. 2012 Nov 21;7(11):e50148. doi: 10.1371/journal.pone.0050148

Figure 4. Titration of lentiviral titers using HT1080 cells.

Figure 4

Harvested lentiviral supernatants were subjected to 10-fold serial dilution for 5 times, and then used to transduce HT1080 cells. These cells were cultivated under Blasticidin selection (5 µg/ml) for 14 days, and stained with crystal violet. The stained colonies in plates were counted and used to calculate the titers of lentiviral vectors, which were presented collectively in Table 1.