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. 2012 Nov 21;7(11):e49387. doi: 10.1371/journal.pone.0049387

Table 1. A list and description of Rab isolates created for the C. elegans ORFeome-based toolkit.

Other Name(s) Sequence Name Rab Subfamily Refseq with Best Match(% Identity) WT DN CA
rab-1 C39F7.4 Rab1 NP_503397.1 (100) IK3-1 IK20-1 PP50-1
unc-108/rab-2 F53F10.4 Rab2 NP_491233.1 (100) GC5-1 GC33-2 SM15-1
rab-3 C18A3.6 Rab3 NP_001021974.1 (100) AP2-1 NG33-1 CG7-1
rab-5 F26H9.6 Rab5 NP_492481.1 (100) PD3-1 NH11-1 MJ21-1
rab-6.1 F59B2.7 Rab6 NP_498993.1 (100) SDS6-1 PRS33-2 SDS34-1
rab-6.2 T25G12.4 Rab6 NP_510790.1 (100) AV3-2 NJ16-1 AV11-1
rab-7 W03C9.3 Rab7 NP_496549.1 (100) MG2-1 SI46-4 SI28-5
rab-8 D1037.4 Rab8 NP_491199.2 (100) MB5-2 DK26-1 MB38-1
rab-10 T23H2.5 Rab10 NP_491857.1 (100) MB10-1 SBA42-1 CN28-1
rab-11.1 F53G12.1 Rab11 NP_490675.1 (100) ZY2-2 PRI8-7 ZY10-1
rab-11.2 W04G5.2 Rab11 N/A OST indicates retention of intron one
rab-14 K09A9.2 Rab14 NP_510572.1 (100) SP6-1 MHB43-1 SP51-1
rab-18 Y92C3B.3 Rab18 N/A ORFeome clone is a rab-18::mlc-3 fusion
rab-19 Y62E10A.9 Rab43 NP_502576.1 (100) NM7-2 KM29-1 DJM14-1
rab-21 T01B7.3 Rab21 NP_495854.1 (100) LAK5-1 JS31-4 DS25-1
aex-6/rab-27 Y87G2A.4 Rab27 NP_493376.1 (100) JP6-1 JP38-1 SAN46-1
rab-28 Y11D7A.4 Rab28 NP_501609.1 (100) SVP6-1 ST21-1 SVP57-2
rab-30 Y45F3A.2 Rab30 NP_499328.1 (100) MEL4-1 TT18-1 SMJ11-1
rab-33 F43D9.2 Rab33 NP_499314.1 (100) TR6-1 TR34-1 MAH25-1
rab-35 Y47D3A.25 Rab35 N/A ORFeome clone contains a rab-27 insert.
rab-37 W01H2.3 Rab37 NP_001041293 (100) RV3-0 ZW10-1 KLD50-1
rab-39 D2013.1 Rab39 N/A ORFeome clone lacks the C-terminus.
CeRabY1/tag-312 C33D12.6 Rab45 NP_508523.1 (96) DVD8-2 DVD36-2 AMT92-13
CaRabY2 4R79.2 Rab44 NP_503120.1 (100) LGK3-2 LGK29-1 AMT92-5
CeRabY3 K02E10.1 orphan N/A OST does not confirm ORF
CeRabY4 F11A5.4 Rab2-like NP_507084.1 (99) KK9-2 AMT92-1 AMT92-9
CeRabY5 F11A5.3 Rab2-like NP_507083.1 (100) EB8-2 MR49-1 PM16-1
CeRabY6 C56E6.2 Rab31/Rab50 N/A ORFeome clone did not grow.
glo-1 R07B1.12 Rab32 N/A ORFeome clone not in the database.
N/A Y71H2AM.12 Rab6-like N/A Not examined
N/A ZK669.5 Rab23-like N/A Not examined

WT, DN and CA clones included in the Rab Toolkit are given isolate names otherwise an explanation for its absence is provided. Subfamily classifications are based on Diekmann et al. 2011 [33] and/or data presented here. The majority of C. elegans rab genes are predicted to have only one splice variant with the following exceptions: WormBase describes two splice variants for rab-3 that code for proteins 233 and 219 amino acids (aa) in length. ORFeome project primers were designed to amplify the shorter isoform only. WormBase describes two splice variants for 4R79.2 (Rab44) that code for proteins 311 aa and 395 aa in length. ORFeome project primers were designed to amplify the longer isoform only. Names listed under “other” are from WormBase or Pereira-Leal and Seabra (2001). Finally, while rab-37 shows 100% identity with the Refseq protein NP_001041293 it contains an additional 5 amino acids at its N-terminus. See text for details.