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. 2012 Nov 21;7(11):e50226. doi: 10.1371/journal.pone.0050226

Figure 4. Factors that significantly predicted the number of large scaffolds.

Figure 4

Among our measures of RNA quality, (A) RNA integrity number (RIN) and (B) OD 260/230 ratio were the strongest predictors of the number of scaffolds ≥1000 bp. (C) Sequencing platform also had a strong effect on number of large scaffolds (P<0.001, Table 2; numbers at the base of bars show sample size), and (D) mass of RNA sequenced had a weak but detectable effect (see Table 2). Note, for most samples we used 20, 30 or 40 µg of total RNA for sequencing, but a few samples used intermediate or lower amounts.