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. 2012 Nov 21;7(11):e46542. doi: 10.1371/journal.pone.0046542

Figure 1. Electrophoresis and immunoblot of medusa cell types.

Figure 1

I – SDS-PAGE (a) and immunoblot (b). a – Coomassie-stained 10% SDS-PAGE: 1 (Mes) – mesoglea of adult A.aurita; 2 (Ect) – ectodermal tissue layer; 3 (Mc) – mesogleal cells; 4 (Gf) – germinal epithelium of female gonad. Mr of marker proteins in kDa are indicated on the left. b – immunoblot of the correspondent lines - Mes, Ect, Mc and Gf. II – AU-PAGE (a) and immunoblot (b). a – Coomassie-stained 7% AU-PAGE: 0 – hialuronidase (pI 9.2) marked with asterisk; 1 (Mes) – mesoglea of adult A.aurita; 2 (Ect) – ectodermal tissue layer; 3 (Mc) – mesogleal cells; 4 (Gf) – germinal epithelium of females' gonad. Arrow marks the start of the separating gel. b – immunoblot. For both immunoblots (b) - primary antibodies RA47 in final dilution 1∶5000; secondary antibodies were antirabbit IgG conjugated with alkaline phosphatase in final dilution 1∶20000 (Sigma).