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Spermatozoids added to medusa eggs in vitro in sea water. a - phase contrast microscope, squash preparation. N – eggs' nucleus, sp – spermatozoids. Scale bar – 10 µm. b – c′ – whole egg in the process of fertilization (non-squashed); b – DAPI staining in grayscale, low magnification; c and c′ - phase contrast and DAPI fluorescence in grayscale, the same field. Scale bar – 50 µm.
