Figure 5. V1801 interferes with Erk signal in NCI-H1975 cells.

(a) NCI-H1975 cells were treated with gefitinib (3 µM) or V1801 (3 µM) for indicated time points, lysed, and Western blot analysis was performed. (b) NCI-H1975 cells were treated with indicated protocols for 12 or 24 h, lysed, and Western blot analysis was performed. (c) NCI-H1975 cells were treated with indicated protocols for 24 h, and apoptotic cell death was evaluated by Annexin V/PI staining and flow cytometry. (d) NCI-H1975 cells were treated for 24 h with BOR and/or V1801, and then assessed by MTT assay. (e) NCI-H1975 cells were treated with V1801 (2 µM), gefitinib (2 µM), BOR (10 nM), or their combinations for indicated time points. The cells were then lysed and subjected to Western blotting using indicated antibodies.