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. 2012 Jul 3;227(4):431–445. doi: 10.1002/path.4013

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Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Myeloid cells associated with tumour cells at the localized tumour and micrometastasis sites. (A) Neutrophils [green; Tg(mpx:GFP)] associated with FGF-T-MAE tumour cells (red) at the localized tumour growth site. (B) Neutrophils (blue; Mpx⁺ histochemical staining) associated with the tumour cells (red) at the micrometastasis site. (C, D) Macrophages (blue; Mpx⁻/L-plastin⁺) associated with the tumour cells (red) at the localized tumour (C) and micrometastasis (D) sites. Data are representative images of > 10 independent, reproducible experiments (each n > 30). Image in (A) was acquired using a Leica MZ16FA stereo microscope (bright-field/fluorescence overlay image); those in (B–D) were acquired using a Leica TCS SPE confocal microscope with a × 20 dry objective

Inline graphic — Myeloid cells associated with tumour cells at the localized tumour and micrometastasis sites. (A) Neutrophils [green; Tg(mpx:GFP)] associated with FGF-T-MAE tumour cells (red) at the localized tumour growth site. (B) Neutrophils (blue; Mpx⁺ histochemical staining) associated with the tumour cells (red) at the micrometastasis site. (C, D) Macrophages (blue; Mpx⁻/L-plastin⁺) associated with the tumour cells (red) at the localized tumour (C) and micrometastasis (D) sites. Data are representative images of > 10 independent, reproducible experiments (each n > 30). Image in (A) was acquired using a Leica MZ16FA stereo microscope (bright-field/fluorescence overlay image); those in (B–D) were acquired using a Leica TCS SPE confocal microscope with a × 20 dry objective