Figure 3. Hypoxia caused reduced cell-ECM adhesion and spreading.

A) Cell-ECM adhesion is diminished in cells grown under hypoxic conditions. A431 cells were grown in normoxia, hypoxia or hypoxia+normoxia. The cells were analyzed for their cell adhesion properties as described in the Materials and Methods. B) Growth under hypoxic conditions led to decreased spreading on collagen-I. (I) A431 cells were grown in normoxia, hypoxia or hypoxia+normoxia conditions. The cells were trypsinized and plated onto coverslips coated with collagen-I. The morphology of the cells was recorded at various time intervals. (II) The surface area of the cells was measured and plotted. C) Growth under hypoxic conditions led to decreased spreading on fibronectin. (I) A431 cells were grown in normoxia, hypoxia or hypoxia+normoxia conditions. The cells were trypsinized and plated onto coverslips coated with fibronectin. The morphology of the cells was recorded at various time intervals. (II) The surface area was measured and plotted.