Figure 4. Hypoxia caused redistribution of vinculin and paxillin.

A) Hypoxia led to reduction of vinculin patches. A431 cells were grown in normoxia, hypoxia or hypoxia+normoxia conditions and probed with anti-vinculin primary antibodies followed by labeled secondary antibodies (Green). The actin cytoskeleton was visualized using Alexa568-Phalloidin (Red). B) Hypoxia led to an increase in paxillin patches. A431 cells grown under normoxia, hypoxia or hypoxia+normoxia conditions were fixed, permeabilized and probed with anti-Paxillin followed by labeled secondary antibody (Green). The actin cytoskeleton was visualized using Alexa568-Phalloidin (Red). C) Hypoxia does not alter the expression of vinculin or paxillin. Cell lysate from A431 cells grown under normoxia (Nx), hypoxia (Hx), hypoxia followed by normoxia (Hx+Nx) or hypoxia+Cetuximab (Hx+D) were analyzed by immunoblotting with anti-paxillin or anti-GAPDH primary antibody.