Figure 7. EGF treatment reduced cell-ECM adhesion.

A) Cell-ECM adhesion is diminished in cells treated with EGF. A431 cells were grown under normoxia (Nx), normoxia+EGF (Nx+E) or normoxia+EGF+Cetuximab (Nx+E+D). The cells were used to carry out cell adhesion assay as described in the Materials and Methods. B) EGF treatment led to loss of vinculin patches. A431 cells were grown as in Panel A and probed with anti-vinculin antibody followed by Alexa488 secondary antibody. The actin cytoskeleton was visualized using Alexa568-Phalloidin. C) EGF treatment does not alter the expression of vinculin. A431 cells were grown under normoxia, normoxia+EGF or normoxia+EGF+Cetuximab. Cell lysate were analyzed by immunoblotting with anti-vinculin or anti-GAPDH primary antibodies.