Table 1. The fifty genes that are most strongly up-regulated in guard cell protoplasts compared with intact guard cells.
| AGI Gene Identifier | SI Intact Guard Cells | SI Guard Cell Protoplasts | Fold Difference | Gene Name/Description |
| At3g46230* | 4.4 | 12.9 | 364.9 | ATHSP17.4 (HEAT SHOCK PROTEIN 17.4) |
| At5g12030* | 4.8 | 12.7 | 236.2 | AT-HSP17.6A (HEAT SHOCK PROTEIN 17.6A) |
| At1g59860* | 5.5 | 12.8 | 164.2 | 17.6 kDa class I heat shock protein) |
| At2g29500* | 4.2 | 11.4 | 147.7 | 17.6 kDa class I small heat shock protein |
| At2g46240* | 4.7 | 11.9 | 144.3 | BAG6 (BCL-2-ASSOCIATED ATHANOGENE 6) |
| At5g12020* | 4.5 | 11.6 | 132.5 | HSP17.6II; 17.6 kDa class II heat shock prot. |
| At4g12400* | 3.9 | 10.6 | 102.8 | stress-inducible protein, putative |
| At1g16030* | 5.4 | 12.1 | 100.9 | Hsp70b (heat shock protein 70B) |
| At4g25200* | 4.3 | 11.0 | 99.8 | ATHSP23.6-MITO (heat shock protein 23.6) |
| At5g48570 | 6.0 | 12.7 | 97.5 | peptidyl-prolyl cis-trans isomerase, putative |
| At5g51440* | 5.9 | 12.4 | 90.7 | 23.5 kDa mitochondrial heat shock protein |
| At1g53540* | 5.6 | 12.0 | 82.5 | 17.6 kDa class I small heat shock protein |
| At3g28740 | 4.4 | 10.6 | 76.5 | CYP81D1; electron carrier/monooxygenase |
| At5g52640* | 6.8 | 13.0 | 73.0 | ATHSP90.1 (HEAT SHOCK PROTEIN 90.1) |
| At1g74310* | 7.7 | 13.9 | 72.3 | ATHSP101 (HEAT SHOCK PROTEIN 101) |
| rpl23 | 5.7 | 11.8 | 66.3 | – |
| At3g12580* | 7.8 | 13.8 | 62.8 | HSP70 (heat shock protein 70) |
| psbH | 5.0 | 10.8 | 56.6 | – |
| psaA | 5.7 | 11.3 | 50.4 | – |
| At3g01830* | 6.7 | 12.3 | 48.5 | calmodulin-related protein, putative |
| At1g54050* | 6.4 | 12.0 | 47.9 | 17.4 kDa class III heat shock protein |
| At1g22810* | 5.7 | 11.2 | 44.9 | AP2 domain transcription factor, putative |
| At2g40350* | 6.0 | 11.4 | 44.2 | DNA binding/transcription factor |
| At2g20560* | 6.1 | 11.4 | 39.4 | DNAJ heat shock family protein |
| At5g25450 | 7.0 | 12.3 | 38.1 | cytochrome C reductase protein, putative |
| At1g06760 | 7.2 | 12.4 | 35.2 | histone H1, putative |
| At1g20693 | 5.6 | 10.7 | 34.3 | HMGB2 (HIGH MOBILITY GROUP B 2) |
| At3g28210 | 6.3 | 11.4 | 32.3 | PMZ; zinc ion binding |
| At3g16050* | 6.9 | 11.9 | 31.0 | A37; protein heterodimerization |
| At5g35320 | 6.6 | 11.6 | 31.0 | hypothetical protein |
| At3g24500* | 7.8 | 12.7 | 30.6 | MBF1C/transcription coactivator |
| At1g55920 | 8.2 | 13.1 | 29.4 | ATSERAT2;1 (serine O-acetyltransferase) |
| At3g55500* | 7.6 | 12.4 | 28.8 | ATEXPA16 (EXPANSIN A16) |
| At5g12340 | 5.3 | 10.1 | 27.5 | hypothetical protein |
| At5g47830 | 5.3 | 10.0 | 26.2 | hypothetical protein |
| psbD | 6.4 | 11.0 | 23.4 | – |
| At2g32190 | 6.9 | 11.5 | 23.2 | hypothetical protein |
| At4g34135 | 8.5 | 13.0 | 22.9 | UGT73B2; UDP-glucosyltransferase |
| At2g02230 | 6.6 | 11.1 | 22.7 | AtPP2-B1 (Phloem protein 2-B1) |
| At3g10020 | 7.3 | 11.8 | 22.6 | hypothetical protein |
| psbE | 7.6 | 12.1 | 22.4 | – |
| At2g15480 | 7.6 | 12.1 | 22.0 | UGT73B5 (UDP-glucosyl transferase 73B5) |
| At2g01180 | 7.1 | 11.5 | 21.5 | ATPAP1; phosphatidate phosphatase |
| At2g19310 | 6.2 | 10.6 | 21.5 | hypothetical protein |
| At1g30070 | 6.8 | 11.2 | 21.3 | SGS domain-containing protein |
| At3g09350 | 8.3 | 12.7 | 21.3 | beta-catenin repeat family protein |
| At2g37430 | 7.0 | 11.4 | 21.0 | zinc finger (C2H2 type) family protein |
| At5g09590* | 7.8 | 12.1 | 20.8 | MTHSC70-2 (MITOCHONDRIAL HSP70 2) |
| ycf10_cemA | 6.2 | 10.5 | 20.3 | – |
| At3g23990* | 6.5 | 10.8 | 19.3 | HSP60 (HEAT SHOCK PROTEIN 60) |
Signal intensities (SI) are normalized log2 values. Asterisks identify stress-responsive genes.