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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Immunol. 2012 Oct 24;189(11):5257–5265. doi: 10.4049/jimmunol.1201905

FIGURE 1.

FIGURE 1

(A) Inhibition of CpG-induced IFN-α production by HIV-1 gp120. Freshly isolated pDCs were treated with CpG-B or CpG-A (as indicated) in the presence or absence of recombinant gp120 (JR-FL, MN or IIIB as indicated). The culture supernatants were collected after 2 days (CpG-B) or 18 h (CpG-A) for IFN-α quantification by ELISA. The black horizontal lines indicate the mean values of data derived using pDCs from six (CpG-B) or five (CpG-A) individual donors. (B) HIV-1 gp120, but not HIV-1 p24, impaired CpG-B-induced IFN-α production by pDCs. The cells were treated with CpG-B in the presence or absence of JR-FL gp120. Culture supernatants were collected after 2 days for IFN-α ELISA. The results shown are the means of duplicate measurements using pDCs from four individual donors. (C) JR-FL gp120 decreases TLR9-mediated, but not TLR7-mediated, IFN-α production. The experimental conditions were similar to those for panel B. The results shown are the means of duplicate measurements using pDCs from three individual donors. NS, not significant.