HIV-1 gp120 inhibits CpG-B-induced pDC maturation. (A) pDCs were treated with CpG-B in the presence or absence of JR-FL gp120 and stained with MAbs against CD40, CD83, CCR7 and HLA-DR. The black curve represents untreated pDCs; green curve, JR-FL gp120-treated pDCs; red curve, CpG-B-treated pDCs; blue curve, CpG-B and JR-FL gp120-treated pDCs. (B) Expression of CD40, CD83, CCR7 and HLA-DR in CpG-B and JR-FL gp120-treated pDCs relative to CpG-B-treated pDCs. The data shown represent the mean values ± SEM obtained using pDCs from at least four individual donors.