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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Immunol. 2012 Oct 24;189(11):5257–5265. doi: 10.4049/jimmunol.1201905

FIGURE 5.

FIGURE 5

gp120 and BDCA-2 cross-linking each suppress TLR9, IRF-7 and BAFF mRNA expression in CpG-B-treated pDCs. (A) pDCs were treated with CpG-B in the presence or absence of JR-FL gp120 for 4 h before TLR9, IRF-7, BAFF and APRIL mRNA levels were quantified by qPCR. One experiment representative of three is shown. (B) The relative expression of TLR9, IRF-7, BAFF and APRIL mRNA is presented, for comparison with CpG-B. The results shown are the mean values ± SEM derived from three independent experiments. (C) pDCs were incubated with anti-BDCA-2 MAb (clone AC144) for 30 min at 37°C, washed and then stimulated with CpG-B for 4 h. The results shown are the mean values ± SEM derived using pDCs from three individual donors. NS, not significant.