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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Immunol. 2012 Oct 24;189(11):5382–5392. doi: 10.4049/jimmunol.1200974

Figure 7. Overexpression of Syk kinase rescues phagocytosis in macrophages with decreased Abl/Arg kinase activity.

Figure 7

(A) RAW264.7 cells transduced with MigR1-myr-FLAG-Syk were treated with GNF-2 followed by incubation with sRBCs for 30 minutes. Scale bar = 10 μm. (B) Phagocytic index was calculated and the results presented as an average from three independent experiments. *, p < 0.05 compared to vehicle-treated cells. (C) Expression of myr-FLAG-Syk in RAW264.7 macrophages was confirmed by Western blotting. (D) Model for Abl family kinase signaling during FcγR-mediated phagocytosis. Upon FcγR ligation, Src family kinases (SFKs) activate Abl family kinases. Src and Abl kinases promote the phosphorylation of Syk, which drives particle internalization. Abl kinases may also modulate the phosphorylation of other substrates to promote actin organization at the phagocytic cup.