Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Immunol. 2012 Oct 26;189(11):5347–5355. doi: 10.4049/jimmunol.1201545

Figure 4. TLR1 602S/S monocytes and macrophages resist mycobacterial inhibition of IFNγ-induced CD64.

Figure 4

A. Primary human monocytes of different TLR1 602 genotypes were incubated with or without M. tuberculosis membrane fraction (Mtb mem) in the presence or absence of IFNγ for 24 hours. Surface levels of monocyte CD64 were assessed by flow cytometry. Bars represent the fold change in CD64 expression versus unstimulated cells (black bars; TLR1 602I/I or I/S donors, gray bars; TLR1 602S/S donors). Error bars represent the standard deviation from at least three different blood donors. Asterisks denote significant differences versus unstimulated values (*p<0.05, **p<0.005). B. Representative histograms of CD64 expression (filled histograms; isotype, dashed histograms; unstimulated cells, black histograms; IFNγ-stimulated cells, gray histograms; co-stimulated with IFNγ and Mtb membrane fraction). C. Primary human monocyte-derived macrophages were stimulated with soluble and particulate TLR1/2 agonists in the presence or absence of IFNγ for 24 hours. Surface levels of CD64 were assessed by flow cytometry. Bars represent the fold change in CD64 expression versus unstimulated cells (black bars; TLR1 602I/I or I/S donors, gray bars; TLR1 602S/S donors). Error bars represent the standard deviation from at least three different blood donors. Asterisks denote significant differences versus unstimulated values (*p<0.05). D. Representative histograms from indicated genotypes: top row (filled histograms; isotype, black histograms; IFNγ-stimulated cells, gray histograms; co-stimulated with IFNγ and Mtb membrane fraction) and bottom row (filled histograms; isotype, black histograms; IFNγ-stimulated cells, gray histograms; co-stimulated with IFNγ and M. avium- Mav).