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. 2012 Nov 21;151(5):964–980. doi: 10.1016/j.cell.2012.10.040

Figure 2.

Figure 2

Piwi/Mael-Mediated TE Silencing Is a Nuclear Process

(A) Cartoon showing protein domains of GFP-tagged Piwi and the residues that have been altered for the mutant analysis.

(B) Confocal images of egg chambers expressing the indicated GFP-Piwi constructs in piwi[1]/piwi[2] background.

(C) Fold changes in steady-state RNA levels of actin5C and indicated TEs from ovaries expressing indicated GFP-Piwi constructs in piwi[1]/piwi[2] background (normalized to piwi[1]/piwi[2]; GFP-Piwi; n = 3; error bars represent SD).

(D) Cartoon showing protein domains of GFP-tagged Mael and the residues that have been altered for the mutant analysis.

(E) Confocal images of egg chambers (left) or OSCs (right) expressing the indicated GFP-Mael constructs (GFP) in wild-type background.

(F) Summary of the rescue experiments (egg laying and egg hatching) with indicated GFP-Mael constructs in mael[391]/mael[Def] background.

(G) Fold changes in steady-state RNA levels of actin5C and indicated TEs from ovaries expressing indicated GFP-Mael constructs in mael[391]/mael[Def] background (n = 3; error bars represent SD).