Figure S6.

Related to Discussion

(A) Shown are normalized density profiles of Pol II ChIP-seq (red), GRO-seq (black), RNA-seq (brown), H3K9me3 ChIP-seq (green) and piRNA-seq (light green) for the indicated OSC knockdowns (left). Shown is the ∼20kb area around the transcriptional start site of the flamenco cluster. Shown are only reads mapping uniquely to the genome but we note that nearly all areas in this window are genome-unique.

(B) Western blot showing protein levels of Armi, Piwi, Lamin, Mael, HP1 and Histone 3 (H3) in cytoplasmic, nucleoplasmic, soluble and insoluble chromatin fractions of OSCs. The relative amount of each fraction loaded per lane (based on fraction volume) is given below. The following antibodies were used: α-Lamin (ADL67.10, DSHB), α-HP1 (C1A9, DSHB) and α-H3 (Abcam, ab1791).