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. 2012 Jan 10;17(12):1261–1271. doi: 10.1038/mp.2011.180

Figure 1.

Figure 1

WT and Mecp2308 iPS cells are pluripotent. (a) WT #3 and HET #4 iPS cell lines express pluripotency markers alkaline phosphatase, Nanog and SSEA-1 by immunocytochemistry. (b) qRT-PCR analysis demonstrates that WT and HET iPS cell lines reactivate endogenous pluripotency loci (mouse mOct4 and mNanog) similar to the J1 ES cell line and also silence exogenous viral reprogramming transgenes (pMXs). The published partially reprogrammed EOS3F-24 (#24) iPS cell line is a positive control for transgene expression. Mouse embryonic fibroblasts are a differentiated cell type-negative control for pluripotency marker expression.15 (c) DAPI-colocalized H3K27me3 foci representing the inactive X-chromosome are absent in WT #3 and HET #4 iPS cells, in comparison with mouse embryonic fibroblasts (arrows). The H3K27me3 silencing mark is not present in all mouse embryonic fibroblasts because they are a mixture of male and female cells. Scale bars: 250 μm.