Figure 3.

In vitro toxicity against a) T. brucei 427 and b) HEK mammalian cells. Wild-type trypanosomes and HEK cells were treated with acivicin (•), 3-bromoacivicin (▪), pentamidine (▾), diminazene (♦), and phenylarsine oxide (○) in 96-well plates at a range of concentrations as described in the Experimental Section. Alamar Blue was added 48 h post-treatment, and fluorescence was used as a measurement of parasite/cell viability. Results are expressed as the mean of each compound’s effective dose that kills 50 % of the cells (i.e., ED₅₀) obtained from duplicates of three independent experiments (n=3); error bars represent standard errors.