Figure 4.

In vivo efficacy of 3-bromoacivicin against T. brucei 427 inoculated in adult female ICR mice (2×10⁴ parasites per mouse in 100 μL per i.p. injection). Mice in groups of three were treated daily for four days (represented by arrows “↓”) with 3-bromoacivicin 50 mg kg⁻¹ (▪) and pentamidine 4 mg kg⁻¹ (♦) given via i.p. injections (100 μL per injection). Untreated mice (▴) acted as negative control. Parasitemia levels were monitored daily by venepunctures, and for cases in which parasitemia reached, or was about to reach ∼10⁸ cells mL⁻¹, the animal(s) were euthanized using Schedule 1, or at the end of procedure. Error bars represent standard deviations between the three animals within each group.