Figure 2.

RNA-blot analyses of the influence of cold acclimation, deacclimation, water stress, and heat shock on the CAP160 steady-state mRNA abundance. A, Acclimation, deacclimation, and water-stress responses. B, Short-term response to low temperature. C, Response to heat shock. The cDNA hybridizes to a major 2.7-kb mRNA. Each lane was loaded with 5 μg of total RNA, and equal loading was verified by ethidium-bromide staining before blotting. Similar results were observed in several hybridization experiments. Details regarding the physiologic parameters for the water-stressed samples have been published elsewhere (Guy et al., 1992; Neven et al., 1993).