Table 1.
Characteristics of wild-type (WT) and ob/ob (OB) mice receiving an AIN-93G diet (AIN) or a sterculic oil-supplemented diet (SO)1.
| P value2 | |||||||
|---|---|---|---|---|---|---|---|
| WT AIN | WT SO | OB AIN | OB SO | d | g | d × g | |
| Body weight (g) | 24.0 ± 0.5 | 25.2 ± 1.0 | 48.6 ± 1.1 | 48.5 ± 0.9 | 0.554 | <0.001 | 0.471 |
| Food intake3 (g/day) | 2.7 ± 0.1 | 2.9 ± 0.1 | 4.1 ± 0.2 | 4.1 ± 0.4 | 0.582 | <0.001 | 0.908 |
| Tissue weights (g) | |||||||
| Gonadal AT4 | 0.4 ± 0.0 | 0.5 ± 0.1 | 3.1 ± 0.1 | 3.8 ± 0.1¥ | <0.001 | <0.001 | 0.005 |
| Subcutaneous AT | 0.3 ± 0.0 | 0.4 ± 0.0 | 3.4 ± 0.1 | 3.5 ± 0.1 | 0.320 | <0.001 | 0.828 |
| Perirenal AT | 0.1 ± 0.0 | 0.1 ± 0.0 | 2.1 ± 0.1 | 2.3 ± 0.1 | 0.375 | <0.001 | 0.541 |
| Liver | 0.8 ± 0.0 | 1.0 ± 0.0 | 3.4 ± 0.1 | 2.8 ± 0.1¥ | 0.031 | <0.001 | <0.001 |
| Liver TG5 (nmol/g wet wt) | 12.0 ± 3.7 | 9.26 ± 2.8 | 25.2 ± 7.3 | 30.2 ± 8.3 | 0.626 | <0.001 | 0.115 |
| Blood glucose (mg/dL) | 75.3 ± 5.7 | 82.4 ± 6.8 | 91.7 ± 6.6 | 94.8 ± 11.2 | 0.202 | <0.05 | 0.552 |
| Plasma insulin (ng/mL) | 0.4 ± 0.0 | 0.3 ± 0.0 | 4.2 ± 0.1 | 6.7 ± 1.6 | 0.454 | <0.001 | 0.471 |
1Data are presented as means ± SEM with n = 6-7 per group.
2Data were analyzed by 2-way ANOVA with diet (SO or AIN) as one factor and the phenotype (WT or OB) as the second factor. P-values are for an effect of diet (d), and effect of the genotype (g), or an interaction between the two (d × g). When a significant interaction between genotype and diet was found individual means were compared within groups by Bonferroni multiple comparisons.
¥ P < 0.05 OB AIN versus OB SO.
3Average daily food intake across the 9 week study.
4AT: adipose tissue.
5TG: triglyceride.