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Sequence of primers, PCR product size, restriction enzymes, and size of digested fragments that are used for screening by PCR-RFLP.
| Mutation | Sequence of primers | PCR product | Restriction | Wild type | Heterozygote | Mutant |
|---|---|---|---|---|---|---|
| (bp) | enzyme | |||||
| MTHFR | F-TGAAGGAGAAGGTGTCTGCGGGA | 198 | Hinf1 | 198 | 198/175/23 | 175/23 |
| C677T | R-AGGACGGTGCGGTGAGAGTG | |||||
|
| ||||||
| MTHFR | F-CTTTGGGGAGCTGAAGGACTACTAC | 163 | MboII | 56/31/30/28/18 | 84/56/31/30/28/18 | 84/31/30/18 |
| A1298C | R-CACTTTGTGACCATTCCGGTTTG | |||||
