Table 2.

Primer pairs used for screening of MTHFR mutations by ARMS-PCR.

Mutation	Forward primer	Reverse primer	PCR
			product (bp)
MTHFR	5-TGC TGT TGG AAG GTG CAA GAT-3	RW 5-GCG TGA TGA TGA AAT CGG-3	226
C677T		RM 5-GCG TGA TGA TGA AAT CGA-3	226
MTHFR	5 -CCTTTGGGGAGCTGAAGGACTACTAC-3	RW 5-CAAAGGACTTCAAAGACAGTC-3	120
A1298C		RM 5-GGTAAAGAACAAAGACTTCAAAGACACTGTG-3	127