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. 2012 Oct 31;2012:792705. doi: 10.1155/2012/792705

Figure 3.

Figure 3

Posttranslational regulation of Ha-Ras and Ki-Ras by hydrogen peroxide in cultured astrocytes. RT-PCR analysis of Ha-Ras and Ki-Ras mRNA in control astrocytes (−) and in astrocytes treated with hydrogen peroxide for 5 min (+) is shown in (a). Immunoblot analysis of Ha-Ras and Ki-Ras in pan-Ras immunoprecipitates from cultures pretreated for 15 min with cycloheximide (CHX, 10 μg/mL) and then exposed to hydrogen peroxide for 5 min is shown in (b). Note that CHX did not affect the rapid increase in Ha-Ras and Ki-Ras levels induced by hydrogen peroxide. The immunoblot has been repeated twice with identical results.