Fig. 2.

Expression analysis of Lesarbohs and their Arabidopsis respiratory burst oxidase orthologues. Transcript abundance analysis for five members of the gene family determined by qRT-PCR in different plant tissues of L. sativum and A. thaliana. Note the similarities in intensity and expression profile between the two species. Averages of three biological replicates ± SE are presented. For the imbibed seed samples, seeds were harvested after 24 h on water in continuous light. Other samples included 5-d-old seedlings (5 d post-imbibition), and mature adult plants harvested 4 weeks post-germination. No AtrbohA transcript was found in any of the stages tested. Transcript abundance was corrected to the abundance of transcripts associated with the standard genes—the constitutive genes Actin 7 (ACT7; At5g09810) and Elongation Factor 1α (EF1α; At5g60390) for Arabidopsis and the corresponding genes (ACT7, HQ436350.1 and EF1α, HS981853.1) for L. sativum. n.d.=not detectable.