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. 2012 Oct 23;63(18):6325–6334. doi: 10.1093/jxb/ers284

Fig. 5.

Fig. 5.

Expression of auxin-related genes in roots and shoots of L. sativum seedlings of wild-type and Lesarboh-RNAi knockdown lines. Transcript abundance for the genes encoding the LOB-domain containing protein (LBD) 16 (A), the auxin-responsive-protein, IAA14 (B) and the auxin-responsive protein IAA5 (C) as determined by qRT-PCR. Stage 1 corresponds to the phenotype shown in Fig. 4B, while stage 2 corresponds to the phenotype shown in Fig. 4C–E. Transcripts in wild-type seedlings were examined under 2,4-D treatment (24 h on 1 µM 2,4-D) and control conditions. Transcript abundance was corrected to the abundance of transcripts associated with the standard genes – the constitutive genes Actin 7 (ACT7; At5g09810) and Elongation Factor 1α (EF1α; At5g60390). Averages of three biological replicates ± SE are presented.