Fig. 3.
Analysis of the transactivation activity of SlDREB. SlDREB and GAL4 DNA-binding domain fusion protein were expressed in the yeast strain AH109. Vectors pGBKT7 and pGBKT7-53+pGADT7-RecT were expressed in yeast as a negative and positive control, respectively. The yeast streak was cultured on SD/–Trp and SD/–Ade/–His/–Trp medium; both contained X-α-gal for assaying another yeast reporter (MEL1) gene. (This figure is available in colour at JXB online.)