Fig. 5.
Effect of Pi deprivation on appearance and shoot biomass accumulation of soil-grown Col-0 and mutant Arabidopsis seedlings. (A) Seedlings were cultivated for 7 d in liquid medium containing 0.2mM Pi, then transplanted into a Pi-deficient soil mix and grown for an additional 14 d. Fertilization occurred twice weekly with 0.25× Hoagland’s medium containing 0 or 2mM Pi (–Pi and +Pi, respectively). Solid bars, 1cm; dashed bar, 0.5cm. (B) Rosette dry weights of soil grown seedlings. All values represent means ±SE of ten different seedlings; asterisks indicate values that were significantly different from those of Col-0 (P <0.01).