ΔFosB levels are not driven by ethanol consumption alone. Hierarchical clustering was performed and the resulting heat map of individual ΔFosB levels and the corresponding 9% ethanol consumption are shown. Green = ΔFosB less than mean, Black = closest to mean, Red = ΔFosB higher than mean. Yellow boxes highlight clusters consisting of mice from control groups, multiple concentration groups, or a mixture. Brain region abbreviations: infralimbic cortex; Cg1, cingulate cortex 1; Cg2, cingulate cortex 2; NAcc core, nucleus accumbens core; NAcc shell, nucleus accumbens shell; LSi, lateral septum intermediate; La, lateral amygdala; Bla, basolateral amygdala; CeC/CeL, central capsular and central lateral amygdala; CeMPV - medial posterioventral portion of the central nucleus of the amygdala; PAG, periaquaductal gray; EW - Edinger-Westphal nucleus; VTA, ventral tegmental area; DR, dorsal raphe; PBN, parabrachial nucleus; NTS, nucleus tractus solitarius.