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. 2012 Apr 30;10:29. doi: 10.1186/1477-5956-10-29

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Copyright ©2012 Aroor et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Quantitative analysis of cytosolic isocitrate dehydrogenase and glutamine synthetase from 2D gels and western immunoblot analysis. Rats were fed ethanol in liquid diet chronically for 4 weeks and then given 3 binge of ethanol and samples collected as in Figure 1. The levels of isocitrate dehydrogenase and glutamine synthetase were determined by western immunoblot. Control represents pair –fed animals given water for binge control. Values are mean ± SE (n = 4 rats). Western blot represents a typical experiment. *significant from control group (p < 0.05); C: Control (pair fed); E: Chronic ethanol; C-B: Control- ethanol binge; E-B: Chronic-ethanol binge.