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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Genesis. 2012 Sep 20;50(11):844–850. doi: 10.1002/dvg.22344

Figure 4.

Figure 4

Confocal acquisition parameters affect image quality. All images DAPI-stained E9.5 mouse embryo. (a) Embryo imaged with optimal parameter settings appears whole and complete. (b) Optical z-axis cropping results when first and last optical sections are not set at the limits of detection of the specimen. (c) Artifact of apparent stacked or layered appearance occurs when the parameters for pinhole size and distance between sections is set with insufficient overlap between optical sections.