Table 1.
Nuclear Stain/Dyes | Conventional microscopy lamp/filter | Confocal microscope excitation laser |
DAPI | Xenon or mercury/UV filter | 405 nm laser |
bisbenzimide (Hoechst) | Xenon or mercury/UV filter | 405 nm laser |
Red Dot 1 (Biotium) | 488, 532, 543, 568, 594, 633, 635 and 647 nm | |
647 nm optimal; | ||
Draq5 (Biostatus) | 488, 514, 568, 633 nm sub-optimal |