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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Genesis. 2012 Sep 20;50(11):844–850. doi: 10.1002/dvg.22344

Table 1.

Examples of nuclear stains that can be used for Pseudo-SEM imaging of vertebrate embryos and embryonic tissues.

Nuclear Stain/Dyes Conventional microscopy lamp/filter Confocal microscope excitation laser
DAPI Xenon or mercury/UV filter 405 nm laser
bisbenzimide (Hoechst) Xenon or mercury/UV filter 405 nm laser
Red Dot 1 (Biotium) 488, 532, 543, 568, 594, 633, 635 and 647 nm
647 nm optimal;
Draq5 (Biostatus) 488, 514, 568, 633 nm sub-optimal