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Examples of confocal z-stack acquisition parameters for E9.5 mouse embryo demonstrating the relationship between pinhole diameter, optical slice thickness and number of optical sections.
| Pinhole diameter | Optical slice thickness | # optical sections | Total imaging depth |
|---|---|---|---|
| 196 | 33.3 μm | 24 | 765 μm |
| 70 | 12.15 μm | 62 | 740 μm |
