Skip to main content
. 2012 Dec;18(12):2148–2156. doi: 10.1261/rna.035725.112

FIGURE 2.

FIGURE 2.

Validation of Asunder, CG4785, CycC, and Cdk8 as required for snRNA 3′-end formation. (A) Images from S2 cells transfected with the U7-GFP reporter after dsRNA treatment targeting Asu or CG4785. (B) Western blot analysis of cell lysates from A. (C) qRT-PCR analysis specific for misprocessed endogenous snRNA from knockdown cells. (D) Western blot analysis of S2 cells treated with dsRNAs targeting CycC or Cdk8 followed by transient transfection with reporters measuring snRNA or histone mRNA 3′-end formation. (E) Western blot analysis of cells cotransfected with U7-GFP reporter and myc-tagged Cdk8 that is wild type or catalytically inactive.