FIGURE 3.

Cyclin C and Cdk8 are not involved in regulating Integrator expression. (A) Western blot analysis of protein levels of Integrator subunits after treating cells with dsRNAs targeting LacZ, CycC, or Cdk8. Cells were also transfected with the U7-GFP reporter to monitor snRNA misprocessing as a functional readout of knockdown of CycC/Cdk8. (B) Quantitative RT-PCR analysis using SYBR Green staining of PCR products. RNA was isolated from S2 cells, treated with the three dsRNAs described in A, and subjected to real-time PCR analysis using amplicons specific to Integrator subunits not tested by Western blot analysis in A. All measurements were normalized to RpS17 signals as a housekeeping internal control. (C) Ethidium bromide staining of agarose gel electrophoresis of the amplicons amplified in B.