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. 2012 Dec;18(12):2148–2156. doi: 10.1261/rna.035725.112

FIGURE 4.

FIGURE 4.

Asunder and CG4785 biochemically associate with the fly Integrator complex. (A) Western blot analysis of Flag-tagged Asunder or CG4785 expression in stable S2 cell lines. (B) IF analysis of stable S2 cell lines expressing Flag-tagged Asunder or CG4785. (C) Western blot analysis of Flag immunoprecipitates from cell lines described in A. Input lanes represent 5% of input, and IP represents 50% of the immunoprecipitate. (D) Directed yeast two-hybrid using fly Integrator subunits and CG4785 demonstrating an interaction with IntS10.