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In vitro reconstitution of m1A58 with recombinant Trmt61B. (A) m1A58 in the tRNALeu(UUR) transcript was detected by primer extension. (B) LC/MS analysis of RNase T1–digested fragments of the tRNALeu(UUR) transcript. Mass chromatograms detecting [M-10H]10− ions for the unmodified 23-mer fragment (positions 54–76, m/z 709.7, and MW 7106.9) and the 23-mer fragment containing a single methyl group (m/z 711.0 and MW 7120.9).
