Figure 6.

Kdm4b acted as a co-factor of C/EBPβ to activate the promoters of the four cell cycle genes. (a) ChIP analysis indicated that Kdm4b was also associated with the promoters of the four cell cycle genes. (b) At 20 h post-induction, soluble chromatin was prepared for ChIPs and Re-ChIPs with the indicated antibodies. Recovery of the four cell cycle gene promoters was analyzed by PCR. (c, d) Interaction between C/EBPβ and Kdm4b. At 20 h post-induction, cell lysates were harvested and immunoprecipitated with antibody against C/EBPβ (c) or Kdm4b (d). Immunoprecipitate complexes were detected by western blotting with the indicated antibodies. The band detected by the C/EBPβ antibody is liver-enriched activator protein (LAP) (34 kDa). (e) 3T3-L1 preadipocytes were transfected with reporter constructs with/out the indicated expression vectors. After 48 h, luciferase activity was analyzed and plotted. *P<0.05