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Validation of chromatin occupancy and mRNA expression of a selected panel of newly identified p53-target genes. (a) ChIP using the anti-p53 antibody DO-1, followed by qPCR, was used to detect relative p53 occupancy in the promoters of 18 selected genes in MCF7 cells treated with nutlin3a, RITA and 5-FU for 8 h. CDKN1A serves as a positive control for p53 binding. (b) The mRNA levels of 18 selected genes were detected by qPCR in MCF7 cells treated with nutlin3a
