Figure 4.

Altered expression of Bcl-2 family proteins in resistant cells. (a) Parental and resistant cells were treated with 5 μM PLX4720 for various time and lysed for western blot analysis on indicated Bcl-2 family proteins. Left: WM793 and WM793-Res cells; Right: M238 and M238-Res cells. (b) Parental and resistant cells were treated with DMSO or 5 μM PLX4720 for 24 h. Total RNA was isolated from cells for qRT-PCR analysis on Bmf. X axis, cell types. Y axis, mean relative Bmf mRNA level (normalized against DMSO-treated parental cells) from three experiments using actin mRNA level as an internal control. Error bars represent standard deviations. *P<0.05, **P<0.01, based on two-tail Student's t-test assuming unequal variance