FIGURE 5.

Trim28 promotes HDAC1/E2F3 and HDAC1/E2F4 interactions. A, control and Trim28 knockdown cells were transfected with the indicated expression plasmids. Whole cell lysates (WCL) of transfected cells were subjected to immunoprecipitation (IP) and then immunoblotting (IB). B, H1299 cells were transfected with the indicated expression plasmids. WCLs of transfected cells were subjected to IP and then IB. C, control and Trim28 knockdown H1299 cells were transfected in triplicate with the indicated promoter-reporter, pRL-TK reporter, and protein expression plasmids. Cells were harvested 48 h after transfection for determination of luciferase levels. Asterisks represent significant p values, as follows: *: p < 0.05, **: p < 0.01, and ***: p < 0.005.