TABLE 1.

PCR primers used in this study

ISCU exon 2–4 DNA probe template	Forward	AAAATCCTAGAAACGTGGGG
ISCU exon 2–4 DNA probe template	Reverse	TGCAGTTTCACGGGAGGAAG
ISCU exon 4A–intron 4 DNA probe template	Forward	TCCAGCAGAGGAGAAAACTCAGC
ISCU exon 4A–intron 4 DNA probe template	Reverse	CCAGGCTTCCTGAAGAATGAGTT
ISCU exon 5 DNA probe template	Forward	CTGGCTGAAGATGCAATCAA
ISCU exon 5 DNA probe template	Reverse	CAGACCAAGGCATTCTACTG
ISCU exon 2–4 T7 RNA probe template	Forward	AAAATCCTAGAAACGTGGGG
ISCU exon 2–4 T7 RNA probe template	Reverse	TAATACGACTCACTATAGGGAGATGCAGTTTCACGGGAGGA
ISCU exon 4A–intron 4 T7 RNA probe template	Forward	AATCTGTGCTGTTTCCAGC
ISCU exon 4A–intron 4 T7 RNA probe template	Reverse	TAATACGACTCACTATAGGGAGACCAGGCTTCCTGAAGAATGAGTT
ISCU exon 2–3 qPCR primer	Forward	GGCTCCAGCATGTGGTGACGTA
ISCU exon 2–3 qPCR primer	Reverse	GCGGAACCACAGCCAAATGT
ISCU exon 4A qPCR primer	Forward	TCCAGCAGAGGAGAAAACTCAGC
ISCU exon 4A qPCR primer	Reverse	CCGACAGGAATACCATCCAGACA
ISCU exon 5 qPCR primer	Forward	AGCAGGCCACACCAGCTGTT
ISCU exon 5 qPCR primer	Reverse	GGAAGCGGCTTCTGAACATCTA
ISCU intron 4 qPCR primer	Forward	TAAGTCCCCACACTATCCTGGC
ISCU intron 4 qPCR primer	Reverse	GTTCCTTAGCCTCTTTGACCCT
MYOD1 qPCR primer	Forward	CGACGGCATGATGGACTACA
MYOD1 qPCR primer	Reverse	TGGGCGCCTCGTTGTAGTAG
MYH1 qPCR primer	Forward	CTAAAACTCCTGGTGCCATGGA
MYH1 qPCR primer	Reverse	TTCCTGCAGATGCGGATGC
ISCU1 ORF cloning primer	Forward	TTAAGCTTCCACCATGGTTCTCATTGACATGAGTG
ISCU2 ORF cloning primer	Forward	ATTAAGCTTCCACCATGGCGGCGGCTGGGGCTTTC
ISCU universal ORF cloning and Myc tagging primer	Reverse	CGCCTCGAGCTACAGATCTTCTTCAGAAATAAGTTTTTGTTCTTTCTTCTCTGCCTCTCCTTTTTTGGG