FIGURE 3.
Cdc7 regulates Cul4-DDB1Cdt2-mediated Tob degradation and DNA damage-induced apoptosis. A, U2OS cells transfected with the indicated siRNAs were irradiated with UV. The cell lysates were analyzed using immunoblotting. B, the scheme of identification of CGK733 targets (left). The two-dimensional gel image, scanned at different wavelengths to visualize spot patterns corresponding to proteins labeled with Cy3 (red) or Cy5 (green), is shown (right). pI values are indicated above. The indicated spots were identified as Cdc7 and HMGB1 by peptide mass fingerprinting. C and D, U2OS cells transfected with the indicated siRNAs were irradiated with UV. The cell lysates were analyzed using immunoblotting. The graphs show the percentages of apoptotic cells with condensed nuclei. Values are shown as the mean ± S.D. from triplicate experiments. (**, p < 0.01).