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Labeling of the C-terminal domain of cav-3. A montage of negatively stained cav-3 oligomers after incubation with Ni-NTA-gold; the MAT tag at the C terminus of cav-3 binds the Ni-NTA-gold conjugate. The gold is visualized under these conditions as electron-dense (black) particles. In each example, a cav-3 nonamer (white) is decorated with only one Ni-NTA-gold conjugate (black). Box size = 326 × 326 Å.
