TABLE 1.

Aligned CBMs identified in the primary sequences of RyR1 and RyR2 labeled 1–6

There are also three distinct CBMs, 7–9, that are unique to RyR2. Motifs 1–6 were aligned to show the degree of similarity. Asterisk indicates fully conserved residue; colon indicates conservation of residue with strongly similar properties; period indicates conservation of residue with weakly similar properties. Capital letters indicate key amino acid residues within the motif. CBM 1 is found in the putative cytosolic assembly, and motifs 2–6 are housed in the transmembrane domain. It can be seen that several of the CBMs in the transmembrane region overlap, e.g. 3–5 in both isoforms.

CBM	Sequence alignment		Region CBM located to within RyR
1	(RyR1)1431–1438,	YyYsvrvF	Cytosolic
	(RyR2)1426–1433,	YyYsvriF
		******:*
2	(RyR1)4712–4720,	FpsnyWdkF	Transmembrane
	(RyR2)4640–4648,	FpnnyWdkF
		**.******
3	(RyR1)4850–4857,	YlYtvvaF	Transmembrane
	(RyR2)4779–4786,	YlYtvvaF
		********
4	(RyR1)4852–4860,	YtvvaFnfF	Transmembrane
	(RyR2)4781–4789,	YtvvaFnfF
		*********
5	(RyR1)4857–4864,	FnFfrkfY	Transmembrane
	(RyR2)4786–4793,	FnFfrkfY
		********
6	(RyR1)5015–5023,	YqercWdfF	Transmembrane
	(RyR2)4944–4952,	YqercWefF
		******:**
7	(RyR2)1445–1453,	WitsdFhqY	Cytosolic
8	(RyR2)4283–4291,	FfssyWsiF	Transmembrane
9	(RyR2)4297–4305,	FvasvFrgF	Transmembrane