FIGURE 2.

EGF signaling inhibits α5β1 integrin-dependent adhesion of DiFi colon cancer cells to fibronectin. A, DiFi cells were seeded onto fibronectin(10 μg/ml)-coated wells for 75 min in the presence of the indicated concentrations of EGF. Cell adhesion was assayed as described in the legend to Fig. 1. B and C, DiFi cells were pretreated for 1 h with the EGFR kinase inhibitor AG1478 (B) or the MEK inhibitor PD184352 (C) at the indicated concentrations prior to assay with or without EGF (10 nm). Error bars indicate S.D. of triplicate samples for one of three representative experiments.