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. 2012 Sep 18;287(48):40457–40470. doi: 10.1074/jbc.M112.418392

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Detection of endogenous S-nitrosylation of apo-S100A1 protein in PC12 pheochromocytoma cell line using the biotin switch method combined with anti-S100A1 Western blot. Lane 1, chemically S-nitrosylated, recombinant human apo-S100A1-NO protein eluted from neutravidin resin after BSM enrichment (rec apo-S100A1-NO BS); lane 2, total protein fraction (control BSM without Asc) before affinity enrichment on neutravidin; lane 3, total protein fraction (full BSM) before affinity enrichment on neutravidin; lane 4, protein fraction unbound to neutravidin (control BSM without Asc); lane 5, protein fraction unbound to neutravidin (full BSM); lane 6, wash fraction (control BSM without Asc); lane 7, wash fraction (full BSM); lane 8, proteins enriched on neutravidin resins (control BSM without Asc); lane 9, proteins enriched on neutravidin resins (full BSM).